Always follow the specific RNeasy kit protocol for RPE volume (typically 500–750 µl per wash) and never reuse buffer. Report prepared for laboratory personnel using Qiagen RNeasy kits. For safety data, consult Qiagen SDS for Buffer RPE (catalog numbers 1018013, 1018019).
| Step | Function | Mechanism | |------|----------|------------| | | Remove chaotropic salts (e.g., guanidine thiocyanate) | Ethanol precipitates RNA; salt contaminants dissolve into ethanol/Tris buffer | | Second wash (RPE) | Remove residual ethanol-soluble inhibitors (e.g., phenol, proteins) | Further purification; low-ionic strength buffer prevents RNA elution prematurely | buffer rpe qiagen