Facs Diva Software -
Always run your single-stain controls using the same voltages as your experiment. Then use Compensation Controls > Calculate – and check the spillover matrix. Any value >1.0 means you have a problem (usually spectral overlap that compensation can't fix). 5. Diva in the Era of Spectral Flow With spectral cytometers (like the Aurora or ID7000) rising in popularity, where does Diva fit? BD's response is FACS Chorus (for the spectral cell sorters) and BD Horizon for newer analyzers. But Diva remains the gold standard for traditional PMT-based, compensation-heavy workflows.
I’ve structured it like a mini-feature article, balancing technical insight with real-world lab appeal. If you’ve ever worked with a BD flow cytometer or sorter, you know the name. You may have whispered it in reverence—or shouted it in frustration. FACS Diva is the proprietary software that powers BD’s digital flow cytometers (from the Canto to the Fortessa, Fusion, and Symphony). But it’s far more than a "control panel." facs diva software